Directed evolution of computer designed enzymes using droplet-based microfluidics

• Project promoter: Vilnius universityInstitute of Biotechnology
• Project partner: Eidgenoessische Technische Hochschule Zuerich
• Implementation period: 01.11.2012–30.04.2016
• Budget: 2.430.458 LTL

Short project description

The aim of the Project: Darwinian evolution is a powerful algorithm that has given rise to the functionally diverse set of proteins present in all living systems. A better understanding of how new enzymes evolve consequently remains an important and challenging task for both academic and industrial needs. Although the active sites of natural enzymes are highly complex, making the design of new or promiscuous protein functions difficult, computation has recently emerged as a potentially powerful strategy for creating protein catalysts with tailored activities and specificities. Because the activities of such artificial enzymes are still modest, we plan to explore the feasibility of optimizing them by combining the latest advances in droplet-based microfluidics technology with the methods of directed evolution. The project aims to deepen understanding of enzyme catalysis and to provide valuable insights into the evolvability of promiscuous protein functions and scaffolds.